If you need to check file is exixt or not on folder. Normally we require to check image is exist or not, video is exists or not etc. In this example i use file_exists() for determine file is available or not in given path. So if you also need check then follow bellow example:
Example:
if(file_exists('./upload/flower.jpg')){
print_r('Image is exist.');
}else{
print_r('Image is not exist.');
}